DETERMINATION OF MERCURY BY LIQUID CHROMATOGRAPHY IN FRESH WATER FISHES USING 2-THIOPHENALDEHYDE-4-PHENYL-3- THIOSEMICARBAZONE

Authors

  • M Y Khuhawar Dr M A Kazi Institute of Chemistry, University of Sindh. Jamshoro, Sindh, Pakistan
  • S N Languani Dr M A Kazi Institute of Chemistry, University of Sindh. Jamshoro, Sindh, Pakistan

Keywords:

Liquid chromatographic technique, Fresh water fishes, Mercury.

Abstract

Co (II), Ag (I) and Hg (II) or Co (II), Ni (II), Fe (II), Cu (II) and Hg (II) are simultaneously extracted as metal chelates
compounds of 2-thiophenaJdehyde-4-pheny1-3-thiosemicarbazone (TAPT) in chloroform. The complexes were separated
from microsorb C-18, 5 urn column when eluted with methanolJacetonitrile/water/aqueous sodium acetate 1m mol or
methanolJacetonitrile/water/sodium acetate (Immol) tetrabutyl ammonium bromide (Immol) with a flow rate of Iml:' and
detection UV at 254 nm. Linear calibrations were made with 10-50 ug ml' and detection limit was 0.4 ug ml', corresponding
to 2 ng/injection in Co and Hg. The method was used for the determination of mercury in surface water fishes. It was
found within 0.125 to 1.18 ug g-! offish muscles with coefficient of variation (C.V) 3.4-5.8%.

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Published

2001-10-22

How to Cite

Khuhawar, M. Y., & Languani, S. N. (2001). DETERMINATION OF MERCURY BY LIQUID CHROMATOGRAPHY IN FRESH WATER FISHES USING 2-THIOPHENALDEHYDE-4-PHENYL-3- THIOSEMICARBAZONE. Biological Sciences - PJSIR, 44(5), 253–256. Retrieved from http://v2.pjsir.org/index.php/biological-sciences/article/view/1941

Issue

Vol. 44 No. 5 (2001): Pakistan Journal of Scientific and Industrial Research

Section

Articles