?ISOLATION AND IDENTIFICATION OF A FLAVONOL GLYCOSIDE USING HIGH SPEED COUNTER CURRENT CHROMATOGRAPHIC TECHNIQUE FROM THE LEAVES OF SALVADORA PERSICA

Authors

  • M Kamil Department of Pharmacognostical Sciences,Zayed Complex for Herbal Research & Traditional Medicine, Ministry of Health, Abu Dhabi, UAE
  • F Ahmad Department of Pharmacognostical Sciences,Zayed Complex for Herbal Research & Traditional Medicine, Ministry of Health, Abu Dhabi, UAE
  • A F Iayaraj Department of Pharmacognostical Sciences,Zayed Complex for Herbal Research & Traditional Medicine, Ministry of Health, Abu Dhabi, UAE
  • C Gunasekhar Department of Pharmacognostical Sciences,Zayed Complex for Herbal Research & Traditional Medicine, Ministry of Health, Abu Dhabi, UAE
  • S Thomas Department of Pharmacognostical Sciences,Zayed Complex for Herbal Research & Traditional Medicine,
  • M Habibullah Department of Pharmacognostical Sciences,Zayed Complex for Herbal Research & Traditional Medicine,Ministry of Health, Abu Dhabi, UAE
  • K Chan Department of Pharmacognostical Sciences,Zayed Complex for Herbal Research & Traditional Medicine,Ministry of Health, Abu Dhabi, UAE

Keywords:

Key words: High speed Counter Current Chromatograph (HSCCC), Salvadora persica, Kaempferol 3-rhamnoside- 7-~-xyloside.

Abstract

This paper introduces the results of separation of a crude flavonoid sample, separated and identified as Kaempferol 3-a-L-
rhamnosyl-7-P-xylopyranoside 1 alongwith Quercetin and Kaernpferolfrom the 10%EtOH extract of the leaves of Salvadora
persica through centrifugal partition chromatography (Pharma-TechCCC-I000 Instrument)using upper phase as mobile phase
and solvent system CHCll; MeOH; EtOH; Hp (5:3:3:4). Detection was carried out at 254nm with flow rate 3ml/min and a
rotational speed 800rpm.Three flavonoidpeakswereresolvedandelutedoutin3.5h.The resultsobtained with thepresent CCC
method are substantially far better than those from the conventional Column chromatography in terms of peak resolution and
separation time, furthermore, separations are much improved.

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Published

2000-08-28

How to Cite

Kamil, M., Ahmad, F., Iayaraj, A. F., Gunasekhar, C., Thomas, S., Habibullah, M., & Chan, K. (2000). ?ISOLATION AND IDENTIFICATION OF A FLAVONOL GLYCOSIDE USING HIGH SPEED COUNTER CURRENT CHROMATOGRAPHIC TECHNIQUE FROM THE LEAVES OF SALVADORA PERSICA. Biological Sciences - PJSIR, 43(4), 255–257. Retrieved from http://v2.pjsir.org/index.php/biological-sciences/article/view/2044