Studies on Antioxidant and Hepatoprotective Activities of Extracts and Active Fraction of Pachypodanthium staudtii (Annonaceae) Stem Bark

Activities of Fraction of Pachypodanthium staudtii

Abstract

This study evaluates the antioxidant and hepatoprotective activities of the stem bark extracts (methanol, PSM and dichloromethane, PSD) and active fraction of Pachypodanthium staudtii. In-vitro antioxidant activity was determined by DPPH, total phenolic content (TPC) and reducing power assay (RPA), while the hepatoprotective activity was verified against CCl4-induced acute hepatotoxicity in rats. Serum biomarkers (ALT, AST, ALP), in-vivo antioxidant (superoxide dismutase, SOD and catalase, CAT) and malondialdehyde (MDA) levels were estimated using standard models. Histopathological studies were carried out on the liver isolates. The result showed that PSM significantly inhibited DPPH (IC50=63.70µg/mL) compared to PSD (IC50=1591.58 µg/mL). PSM showed a higher phenolic content of 2536.26 mgGAE/g compared to 1916.78 mgGAE/g for PSD. Fraction A showed a phenolic content of 553.01 mgGAE/g. Significant (P<0.05) decrease in hepatic biomarkers (ALT, AST, ALP) was observed at varying degrees in PSM and PSD treated groups compared to CCl4-control group. Fraction A showed significant (P<0.05) effect on hepatic biomarkers (ALT, AST, ALP), in vivo antioxidant (SOD, CAT) and MDA levels compared to the CCl4-control group. Histopathology studies revealed restoration of liver architecture and healing from CCl4 intoxication in the liver.