High Frequency In vitro Propagation of Polianthes tuberosa

Authors

  • Muhammad Saeed Ahmad Plant Biotechnology Laboratory, Food and Biotechnology Research Centre, PCSIR Laboratories Complex, Ferozepure Road, Lahore-54600, Pakistan
  • Tauqeer Ahmad Plant Biotechnology Laboratory, Food and Biotechnology Research Centre, PCSIR Laboratories Complex, Ferozepure Road, Lahore-54600, Pakistan
  • Nasreen Zaidi Plant Biotechnology Laboratory, Food and Biotechnology Research Centre, PCSIR Laboratories Complex, Ferozepure Road, Lahore-54600, Pakistan
  • Idrees Ahmad Nasir Plant Biotechnology Laboratory, Food and Biotechnology Research Centre, PCSIR Laboratories Complex, Ferozepure Road, Lahore-54600, Pakistan imtauqeer@yahoo.com

Keywords:

L-arginine, clonal propagation, Polianthes tuberosa, tuberose plant

Abstract

Calli induced on MS medium supplemented with 10 mM a-naphthaleneacetic acid (NAA) grew extensively when cultured on MS medium modified with 4 mM 2,4-dichlorophenoxyacetic acid (2,4-D), producing on an average four shoots per callus culture. The addition of 1 mM L-arginine in the culture media enhanced the induction rate upto 10 shoots per callus culture in 12 weeks. When 2-3 cm long regenerated shoots were replanted on MS medium with 20

mM 6-benzylyadenine (BA) and 4 ?M 2,4-D, shoots the proliferated at the cut ends. Floral axis buds produced 3-4 cm long multiple shoots on NAA and BA. New shoots regenerated from the calli produced at the base of shoots subcul- tured on 10 mM NAA. Repetition of shoot development, callus formation, and again shoot formation on 10 mM NAA and 2 mM BA greatly increased the number of plants from single shoots. Eighty five percent bulb explants produced 290 shoots in 12 weeks directly on 15 mM BA and 5 mM NAA. The somatic psuedoembryos formed in the calli were dormant.

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Published

2006-10-23

How to Cite

Ahmad, M. S., Ahmad, T., Zaidi, N., & Nasir, I. A. (2006). High Frequency In vitro Propagation of Polianthes tuberosa. Biological Sciences - PJSIR, 49(5), 344–348. Retrieved from https://v2.pjsir.org/index.php/biological-sciences/article/view/1245