Antioxidant and Antidiabetic Potential of Saponin Fraction Isolated from Moringa oleifera Leaves

Antioxidant Potential of Saponin Fractions

Authors

  • Fatma Hussain Clinico-Medical Biochemistry Laboratory (CMBL), Department of Biochemistry, Faculty of Sciences, University of Agriculture, Faisalabad-38040, Pakistan
  • Fatima Ikram Clinico-Medical Biochemistry Laboratory (CMBL), Department of Biochemistry, Faculty of Sciences, University of Agriculture, Faisalabad-38040, Pakistan

DOI:

https://doi.org/10.52763/PJSIR.BIOL.SCI.63.2.2020.86.92

Keywords:

Moringa oleifera, antioxidant activity, antidiabetic activity, saponin fraction

Abstract

To assess total phenolic contents, total flavonoid contents (TPC, TFC), DPPH scavenging and antidiabetic (antiglycation and alpha amylase hindrance) activities of Moringa oleifera (MO) leaves, bio- assay guided fractionation was used with thin layer chromatography profile to isolate saponin fraction (SF). The TPC and TFC were 9.7-12.8 GAE and 5.3-9.3 CE (g/100 g dry weight), respectively and aqueous and methanol fractions were more effective. DPPH scavenging activity ranged 69.5-83.1%. SF displayed noteworthy antioxidant ability (p<0.046) and it was leading inhibitor of glycation (p<0.041) after MeOH with 48.1% restriction. Enzyme inhibition by MO fractions was negligible (3.4-9.2%) and it was less impressive by SF as compared to MeOH extract. Percent reduction in enzyme activity by SF was non- significant (p>0.198), nullifying its concentration dependent impact.

 

 

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Published

2020-07-15

How to Cite

Hussain, F., & Ikram, F. (2020). Antioxidant and Antidiabetic Potential of Saponin Fraction Isolated from Moringa oleifera Leaves: Antioxidant Potential of Saponin Fractions. Biological Sciences - PJSIR, 63(2), 86–92. https://doi.org/10.52763/PJSIR.BIOL.SCI.63.2.2020.86.92

Issue

Vol. 63 No. 2 (2020): Pakistan Journal of Scientific and Industrial Research Series B: Biological Sciences

Section

Articles