Rose Bengal and Mercaptoethanol Tests for the Diagnosis of Brucella abortus Biotype-1 Infection in Sprague-Dawley Rats

Authors

  • M. S. Rahman College of Veterinary Medicine, Chonbuk National University, Jeonju 561 756, South Korea
  • B. K. Baeka Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

Keywords:

brucellosis, Brucella abortus biotype-1, Sprague-Dawley rats, Rose Bengal test, mercaptoethanol test

Abstract

Rose Bengal test (RBT) and mercaptoethanol test (MET) were conducted for the diagnosis of Brucella abortus biotype-1 infection. For this purpose, female Sprague-Dawley (S-D) rats were subcutaneously infected at the dose rate of 1.0×109 colony forming unit suspension of B. abortus in physiological saline. The S-D rats were monitored at regular intervals using serological and bacteriological methods. The reciprocal antibody titer after the first week was 1: 800 with RBT, whereas it was 1: 400 with MET. The reciprocal antibody titer of 1: 25 through RBT and 1:100 through MET, respectively, were noted in infected rats of 24 weeks post-infection. Bacteremia was detected untill 24 weeks post-infection. RBT, using B. abortus strain S1119-3 whole cell antigen, appears to be a potential candidate, as a useful diagnostic method, for brucellosis detection in rats.

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Published

2005-12-21

How to Cite

Rahman, M. S., & Baeka, B. K. (2005). Rose Bengal and Mercaptoethanol Tests for the Diagnosis of Brucella abortus Biotype-1 Infection in Sprague-Dawley Rats. Biological Sciences - PJSIR, 48(6), 407–411. Retrieved from https://v2.pjsir.org/index.php/biological-sciences/article/view/1434

Issue

Vol. 48 No. 6 (2005): Pakistan Journal of Scientific and Industrial Research

Section

Articles