Dietary Benefits of Baphia nitida Stem Bark and Antimicrobial Effect on Some Pathogens

Dietary Benefits of Baphia nitida Stem Bark

  • Parker Joshua Elijah Department of Biochemistry, University of Nigeria, Nsukka, Nigeria
  • Bennett Chima Nwangum Department of Biochemistry, University of Nigeria, Nsukka, Nigeria
  • Victor Eshu Okpashi Department of Biochemistry, Cross River University of Technology, Calabar, Nigeria
  • Austin Ike Chukwunonyelum Centre for Environmental Management and Control Unit, University of Nigeria, Enugu
  • Kenne Michel Tchimene Department of Pharmacognosy & Environmental Medicine, University of Nigeria, Nsukka
  • Orhonigbe Innocent Ogheneovo eDepartment of Biochemistry, Delta State University, Abraka, Delta State
Keywords: antimicrobial, Baphia nitida, nutrition, gram-positive, gram-negative

Abstract

The need to find a broad-based nutritional and antimicrobial therapy gave impetus to this investigation. The crude extracts of Baphia nitida stem bark were used to check for antimicrobial effect on selected micro-organisms for possible nutritional and therapeutic application. The stem bark components were extracted with four solvent systems using maceration. A synthetic drug gentamycin - 80 mg/mL (2 mL) was dissolved in 20 mL of distilled water to obtained 8 mg/mL, was used as a control. The crude extract exhibited an antibacterial effect on gram-positive organisms (Staphylococcus aureus and Bacillus cereus), and gram-negative organism (Pseudomonas aeruginosa, and Escherichia coli) at 2 mg/mL. These showed competitiveness with the 8 mg/mL of the control drug. The normal hexane fraction had an effect on Staphylococcus aureus but showed no effect on other bacteria. Minimum inhibitory concentration (MIC) showed a dose-dependent decrease in the effect for the crude extract. Ethyl acetate and methanol fraction had an effect on Staphylococcus aureus, Bacillus cereus, Pseudomonas aeruginosa, and Escherichia coli at varying concentrations - 20, 10, and 5 mg/mL. the initial screening of the Baphia nitida stem bark revealed alkaloids in the methanol-methylene chloride crude extract and methanol fraction only. Other solvents showed the alkaloids, flavonoids, saponins, terpenoids, glycosides, hydrogen cyanides and steroids respectively. Therefore, the presence of the phytochemicals implicates its antimicrobial effect and the basis for its efficacy as antimicrobial therapy.

 

 

Published
2020-11-12
How to Cite
Elijah, P. J., Nwangum, B. C., Okpashi, V. E., Chukwunonyelum, A. I., Tchimene, K. M., & Ogheneovo, O. I. (2020). Dietary Benefits of Baphia nitida Stem Bark and Antimicrobial Effect on Some Pathogens. Biological Sciences - PJSIR, 63(3), 135-141. Retrieved from https://v2.pjsir.org/index.php/biological-sciences/article/view/1494