Evaluation of Phyllanthus niruri L. from Malaysia for In-vitro Anti-Urolithiatic Properties by Different Solvent Extraction

Properties of Anti-Urolithiatic

  • Muhammad Tayyab Gul Faculty of Sciences and IT, Department of Chemical and Life Sciences, Qurtuba University of Science and Information Technology, Peshawar, 25000, Pakistan
  • Norhayati Muhammad Centre of Research for Sustainable Uses of Natural Resources (CoR-SUNR), Universiti Tun Hussein Onn Malaysia (UTHM), Parit Raja, 86400 BatuPahat, Johor, Malaysia
  • Aslia Natasha Pauzi Faculty of Applied Sciences and Technology, Universiti Tun Hussein Onn Malaysia (UTHM), Pagoh Educational Hub, KM 1, Jalan Panchor, 84600 Muar, Johor, Malaysia
  • Mohd Fadzelly Abu Bakar Centre of Research for Sustainable Uses of Natural Resources (CoR-SUNR), Universiti Tun Hussein Onn Malaysia (UTHM), Parit Raja, 86400 BatuPahat, Johor, Malaysia
  • Balkis A. Talip Centre of Research for Sustainable Uses of Natural Resources (CoR-SUNR), Universiti Tun Hussein Onn Malaysia (UTHM), Parit Raja, 86400 BatuPahat, Johor, Malaysia
  • Norazlin Abdullah Centre of Research for Sustainable Uses of Natural Resources (CoR-SUNR), Universiti Tun Hussein Onn Malaysia (UTHM), Parit Raja, 86400 BatuPahat, Johor, Malaysia
  • Nur Fazira Abdul Rahim Faculty of Applied Sciences and Technology, Universiti Tun Hussein Onn Malaysia (UTHM), Pagoh Educational Hub, KM 1, Jalan Panchor, 84600 Muar, Johor, Malaysia
  • Wan Nur Ain Syukriah Faculty of Applied Sciences and Technology, Universiti Tun Hussein Onn Malaysia (UTHM), Pagoh Educational Hub, KM 1, Jalan Panchor, 84600 Muar, Johor, Malaysia
  • Wan Marzuki Faculty of Applied Sciences and Technology, Universiti Tun Hussein Onn Malaysia (UTHM), Pagoh Educational Hub, KM 1, Jalan Panchor, 84600 Muar, Johor, Malaysia
  • Laily B. Din School of Chemical Sciences and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, UKM Bangi 43600, Selangor D.E., Malaysia
  • Nazlina Ibrahim School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia,UKM Bangi 43600, Selangor D.E., Malaysia
Keywords: crystallization, dissolution, nucleation, P. niruri, anti-urolithiatic.

Abstract

 

The Phyllanthus niruri is traditionally used for curing of kidney disorders and urinary stones in Malaysia. Hence the current work was aimed to evaluate the effect of different solvents extract (n- hexane, ethyl acetate, methanol and water) of P. niruri for in vitro anti-urolithiatic properties in terms of inhibition activity on CaOx by using the rate of CaOx aggregation assay and dissolution of calcium oxalate (CaOx) crystal by using titrimetry method. Cystone was used as positive control. The effects of cystone on slope of nucleation and aggregation as well as growth of CaOx were evaluated spectrophotometrically. The highest yield percentage of P.niruri was occupied by methanol (5.74 %). The maximum inhibition against aggregation of CaOx crystals was also occupied by methanol (66.67 % ± 1.61) and was comprised with alkaloid, steroid, terpenoid and tannin. Dissolution effect on calcium oxalate crystals indicates that the aqueous extracts of P. niruri was found to be more effective in dissolution of CaOx with 63.33 %   ± 1.44. P. niruri significantly (P < 0.05) inhibited the slope of nucleation and aggregation of CaOx crystallization, and reduced the crystal density. The results of the present study confirmed that P. niruri leaves can be used as remedial mediator for urolithiasis. However, further studies are required for isolation and identification of active constituents and their in-vivo confirmation.

 

Published
2021-03-03
How to Cite
Gul, M. T., Muhammad, N., Pauzi, A. N., Bakar, M. F. A., Talip, B. A., Abdullah, N., Abdul Rahim, N. F., Syukriah, W. N. A., Marzuki, W., Din, L. B., & Ibrahim, N. (2021). Evaluation of Phyllanthus niruri L. from Malaysia for In-vitro Anti-Urolithiatic Properties by Different Solvent Extraction. Biological Sciences - PJSIR, 64(1), 81-86. Retrieved from https://v2.pjsir.org/index.php/biological-sciences/article/view/1721