Production and Characterisation Partial Lipase of Bacillus halodurans Cml Mutant for Biodetergen

Lipase of Bacillus halodurans

  • Arina Aisyah Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas of Indonesia, Depok-16424, Indonesia
  • Trismilah Trismilah Agro Industrial and Biomedical Technology Development Laboratory, Agency for the Assessment and Application of Technology, Serpong-15314, Indonesia
  • Wibowo Mangunwardoyo Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas of Indonesia, Depok-16424, Indonesia
  • Dadang Suhendar Agro Industrial and Biomedical Technology Development Laboratory, Agency for the Assessment and Application of Technology, Serpong-15314, Indonesia
Keywords: B. halodurans, CM1 mutant, characterisation, lipase

Abstract

 

This study aims to produce lipase of the Bacillus halodurans CM1 mutant and its assess partial characteristics, performed in Bora and Bora modified medium. The purification was conducted using Ultrafiltration (UF), ammonium sulfate (AS) and polyethylene glycol (PEG). Results revealed that the highest purity lipase of B. halodurans CM1 mutant was 1.49-fold from the UF-AS-dyalisis, with a molecular weight of 35.7-37.4 KDa. The optimum condition of lipase enzyme was achieved at pH 7 and temperature 50 °C, relatively stable at pH 7-8 and temperature 30-70 °C. Mg2+, Ca2+, Zn2+, Mn2+, Fe2+ and K+ ions of concentrations, 1 mM to 10 mM increased enzyme lipase activity. The Km value was 0.23 mg/mL and Vmax 4.07 U/mL. Lipase was stable with the addition of a detergent concentration of 1-2% (69.60-57.10%), and with the  washing test, the enzyme capable of hydrolyzing oil on cloth is 8.40%.

 

 

Published
2021-03-03
How to Cite
Aisyah, A., Trismilah, T., Mangunwardoyo, W., & Suhendar, D. (2021). Production and Characterisation Partial Lipase of Bacillus halodurans Cml Mutant for Biodetergen. Biological Sciences - PJSIR, 64(1), 87-93. Retrieved from https://v2.pjsir.org/index.php/biological-sciences/article/view/1722