In vitro Studies of the Utilization of Industrially Important Substrates by Lactobacillus sp. AAF-1 Isolated from Coconut Water

Utilization of Industrial Substrates by Lactobacillus sp. AAF-1

Authors

  • Ayesha Siddiqui Department of Biotechnology, Jinnah University for Women, Karachi-74600, Sindh, Pakistan
  • Ayisha Aman Department of Biotechnology, Jinnah University for Women, Karachi-74600, Sindh, Pakistan
  • Syeda Areej Fatima Department of Biotechnology, Jinnah University for Women, Karachi-74600, Sindh, Pakistan
  • Aliya Riaz Department of Biochemistry, Jinnah University for Women, Karachi-74600, Sindh, Pakistan
  • Rashida Rahmat Zohra Department of Biotechnology, Jinnah University for Women, Karachi-74600, Sindh, Pakistan
  • Suad Naheed Department of Biotechnology, Jinnah University for Women, Karachi-74600, Sindh, Pakistan

DOI:

https://doi.org/10.52763/PJSIR.BIOL.SCI.65.1.2022.62.66

Keywords:

amylase, beta galactosidase, cellulase, coconut, Lactobacillus, protease

Abstract

Coconut water (Cocos nucifera L.) possesses natural hydrating qualities, functional health  properties and various nutritional benefits. Lactobacillus species AAF-1 has been isolated and purified from coconut water. The isolated culture was screened for the production of extracellular secreted enzymes having industrial value. Growth of the Lactobacillus sp. AAF-1 on MRS agar containing respective substrates showed that the Lactobacillus sp. AAF-1 is the potential producer of amylase, protease, cellulase and beta galactosidase. Further cell free filtrates of the Lactobacillus sp. AAF-1 showed large zone of hydrolysis of starch, gelatin, cellulose and lactose that reflected the extracellular production of the above enzymes in culture medium at 50 °C. It has been observed that activities after 72 h of incubation in cell free filtrates of protease, amylase, cellulase and beta galactosidase as the zone of respective substrate hydrolysis measured as 10, 8, 6 and 10 mm respectively. Thus, Lactobacillus sp. AAF-1 could be the potential producer of the enzymes having industrial value.

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Published

2022-02-24

How to Cite

Siddiqui, A., Aman, A., Fatima, S. A., Riaz, A., Zohra, R. R., & Naheed, S. (2022). In vitro Studies of the Utilization of Industrially Important Substrates by Lactobacillus sp. AAF-1 Isolated from Coconut Water: Utilization of Industrial Substrates by Lactobacillus sp. AAF-1. Biological Sciences - PJSIR, 65(1), 62–66. https://doi.org/10.52763/PJSIR.BIOL.SCI.65.1.2022.62.66