Solid State Fermentation of Bacillus amyloliquefaciens SSST for the Production of a-amylase

Production of a-Amylase

Abstract

 a-Amylase is an industrially important enzyme that catalyses the hydrolysis of starch to give products like glucose and maltose. Amylase producing strain was isolated using starch agar screening medium and was identified as Bacillus amyloliquifaciens using 16S rRNA gene analysis. Optimization  of a-amylase production by Bacillus amyloliquefaciens S8TS was done using technique of solid state fermentation (SSF). Various agro industrial substrates (wheat straw, rice husk, wheat porridge, wheat bran) were used for a-amylase production but wheat bran gave highest a-amylase activity (3.65 U/mg) when moistened with 10 mL diluent (D-3) at pH 7.0, temperature 37 °C with 10% vegetative inoculum after 72 h of incubation.