Protective Effects of Cyphomandra betacea Extract Against Hydrogen Peroxide-induced Oxidative Stress in 3T3 Cells and Synergistic Anticancer Properties in HepG2 and MDA-MB-231 Cell Lines

Anticancer Properties of Cyphomandra betacea

Authors

  • Maisarah Abdul Mutalib International Medical School, Management and Science University, University Drive, Off Persiaran Olahraga, Section 13, 40100 Shah Alam, Selangor, Malaysia
  • Asmah Rahmat Faculty of Science, Technology and Human Development, Universiti Tun Hussein Onn Malaysia (UTHM), 86400 Parit Raja Batu Pahat, Johor, Malaysia
  • Muhammad Shuaib Khan Faculty of Veterinary and Animal Sciences,Gomal University D.I.Khan, Pakistan
  • Ali Zaman Faculty of Veterinary and Animal Sciences,Gomal University D.I.Khan, Pakistan
  • Muhammad Inamullah Malik Faculty of Veterinary and Animal Sciences,Gomal University D.I.Khan, Pakistan
  • Khalid Muhammad Faculty of Veterinary and Animal Sciences,Gomal University D.I.Khan, Pakistan
  • Shabnam Razzaq Khan Institute of Research and Education, Gomal University, D.I. Khan, Pakistan
  • Shomaila Noreen Malik Department of Gaynocology and Obstetrics, District Teaching Hospital, D.I.Khan, Pakistan
  • Saqib Ali Rustam Faculty of Veterinary and Animal Sciences,Gomal University D.I.Khan, Pakistan

Keywords:

chemotherapeutic, radiation therapy, hydrogen peroxide, Cyphomandra betacea, synergistic

Abstract

Using the MTT 3-(4, 5-dimethylthiazol-2yl-2, 5-diphenyltetrazolium bromide) assay induced by hydrogen peroxide (H2O2) and the ability to increase the cytotoxicity of doxorubicin in HepG2 and MDA-MB-231 cells, the protective effects of ethanolic extract of tamarillo were assessed in the current study. The cells were pre-treated with tamarillo ethanolic extract at 10 g/mL for 4 h before being exposed to 0.6 mM H2O2 for 3 h showed the greatest protection, with a significant increase (P 0.05) in cell viability of 79% compared to the control (57%). The results support a role that cyto-protective effect of tamarillo and doxorubicin which low in toxicity opens a new possible approach for possible future use in conventional chemotherapeutic drugs and radiation therapy.

 

 

Downloads

Published

2024-07-03

How to Cite

Maisarah Abdul Mutalib, Asmah Rahmat, Muhammad Shuaib Khan, Ali Zaman, Muhammad Inamullah Malik, Khalid Muhammad, Shabnam Razzaq Khan, Shomaila Noreen Malik, & Saqib Ali Rustam. (2024). Protective Effects of Cyphomandra betacea Extract Against Hydrogen Peroxide-induced Oxidative Stress in 3T3 Cells and Synergistic Anticancer Properties in HepG2 and MDA-MB-231 Cell Lines: Anticancer Properties of Cyphomandra betacea. Biological Sciences - PJSIR, 67(2), 167–173. Retrieved from https://v2.pjsir.org/index.php/biological-sciences/article/view/3072

Issue

Vol. 67 No. 2 (2024): Pakistan Journal of Scientific and Industrial Research Series B: Biological Sciences

Section

Articles