Effect of Physical, Chemical and Physiochemical Treatments of Surface Sterilisation on Medicinal Plants Salvadora persica and Solanum surattense for In-vitro Propagation

Abstract

Effect of various surface sterilisation treatments on medicinal plants Salvadora persica and
Solanum surattense has been studied for in-vitro propagation. Surface sterilisation treatment was applied
by physical, chemical and physiochemical means. Chemical sterilisation was done with sodium hypochlorite,
physically with sonication and physiochemically with combination of both. Sodium hypochlorite alone
was found to be effective for smooth textured plant i.e., Salvadora persica, whereas for rough textured
plant Solanum surattense a combination of both physiochemical means yielded good results. However,
sonication alone did not render the effective way of surface sterilisation. Moreover, for Salvadora persica,
10% sodium hypochlorite was effective in eliminating bacterial or fungal growth whereas in Solanum
surattense, a concentration of 15% sodium hypochlorite proved to be effective. In general, best results
were achieved in the combined treatment by physiochemical means.