Production, Partial Purification and Characterization of Lipase from Aspergillus flavus KUF108

Lipase from Aspergillus flavus KUF108

Authors

  • Muthusamy Palaniswamy Department of Microbiology, School of Life Science, Karpagam University, Coimbatore-641 021, Tamil Nadu, India
  • Thamaraichelvan Rajeswari Department of Microbiology, School of Life Science, Karpagam University, Coimbatore-641 021, Tamil Nadu, India
  • Chidambaram Kulandaisamy Venil Department of Microbiology, School of Life Science, Karpagam University, Coimbatore-641 021, Tamil Nadu, India
  • Krishnan Nathiya Department of Microbiology, School of Life Science, Karpagam University,Coimbatore-641 021, Tamil Nadu, India
  • Paulraj Joyruth Department of Microbiology, School of Life Science, Karpagam University, Coimbatore-641 021, Tamil Nadu, India

Keywords:

Aspergillus flavus, lipase, solid state fermentation, agro-industrial waste, enzymatic characterisation

Abstract

Fungi isolated from oil contaminated soils were screened for exogenous lipolytic activity. Optimization of fermentation conditions such as substrate, temperature, pH, moisture content, incubation period, carbon source, nitrogen source and metal ions for maximum lipase production was examined under solid state fermentation by the local isolate of Aspergillus flavus KUF108. Purification of crude enzyme was carried out by ammonium sulphate precipitation, dialysis and DEAE cellulose column chromatography. The lipase was found to be active at pH 5 and 50 °C, and stable between pH 5-6 and 40-60 °C. The apparent molecular weight of purified enzyme was 44 kDa.

 

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Published

2010-10-25

How to Cite

Palaniswamy, M., Rajeswari, T., Venil, C. K., Nathiya, K., & Joyruth, P. (2010). Production, Partial Purification and Characterization of Lipase from Aspergillus flavus KUF108: Lipase from Aspergillus flavus KUF108. Biological Sciences - PJSIR, 53(5), 258–264. Retrieved from https://v2.pjsir.org/index.php/biological-sciences/article/view/474

Issue

Vol. 53 No. 5 (2010): Pakistan Journal of Scientific and Industrial Research

Section

Articles