Purification and Characterization of Bacteriocin Like Substance Produced from Bacillus lentus with Perspective of a New Biopreservative for Food Preservation

Purification of BLIS from B. lentus

Authors

  • Nivedita Sharma Department of Basic Sciences, Microbiology Research Laboratory, Dr. Y.S. Parmar Univerisy of Horticulture and Forestry, Nauni-Solan (H.P.) 173 230, India
  • Ambika Attri Department of Basic Sciences, Microbiology Research Laboratory, Dr. Y.S. Parmar Univerisy of Horticulture and Forestry, Nauni-Solan (H.P.) 173 230, India
  • Neha Gautam Department of Basic Sciences, Microbiology Research Laboratory, Dr. Y.S. Parmar Univerisy of Horticulture and Forestry, Nauni-Solan (H.P.) 173 230, India

Keywords:

bacteriocin like inhibitory substance, BLIS, B. lentus, antimicrobial activity, biopreservative

Abstract

Molecular weight of bacteriocin like substance (BLIS) of a new strain of Bacillus lentus 121 was found to be approximately 11 kDa. Purification of BLIS was attained by single step gel exclusion chromatography. BLIS was characterized by studying the inhibitory spectrum. It was active at broad pH range, high temperature and high NaCl concentration and showed sensitivity to proteolytic enzymes like trypsin, a-chymotrypsin and papain, the characters desirable for food preservation. BLIS extended the shelf stability of milk upto 21 days as a biopreservative.

 

 

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Published

2009-08-24

How to Cite

Sharma, N., Attri, A., & Gautam, N. (2009). Purification and Characterization of Bacteriocin Like Substance Produced from Bacillus lentus with Perspective of a New Biopreservative for Food Preservation: Purification of BLIS from B. lentus. Biological Sciences - PJSIR, 52(4), 191–199. Retrieved from https://v2.pjsir.org/index.php/biological-sciences/article/view/547

Issue

Vol. 52 No. 4 (2009): Pakistan Journal of Scientific and Industrial Research

Section

Articles