The Suitable COI Marker for Lobster of Genus Panulirus

COI Markers for Panulirus Lobster

Authors

  • Achmad Farajallah Department of Biology, Faculty of Mathematics and Natural Science, Bogor Agricultural University,Jl. Raya Dramaga Bogor 16680, West Java, Indonesia
  • Bintang Kusuma Tirtaningsih Handayani Department of Biology, Faculty of Mathematics and Natural Science, Bogor Agricultural University,Jl. Raya Dramaga Bogor 16680, West Java, Indonesia
  • Yusli Wardiatno Department of Aquatic Resources Management, Faculty of Fisheries and Marine Sciences, Bogor Agricultural University, Indonesia

DOI:

https://doi.org/10.52763/PJSIR.BIOL.SCI.62.2.2019.111.115

Keywords:

crustacea, DNA barcode, genetic distance, genetic marker, mutation rates, Panulirus

Abstract

This study was conducted to analyze the COI (cytchrome oxidase subunit-1) sequences of members of the genus Panulirus, and determine the best region to use for species identification through DNA barcoding. Six samples were collected randomly from five sites in Indonesia. The three lobster species collected were P. femoristriga (Padang), P. homarus (Malang), and P. versicolor. A total of 1,516 sequences were analyzed using the software MEGA 6 for covering alignment, nucleotide variation, mutation
rates, and genetic distance. Samples classified in the upper region (UR) consisted of 25 Panulirus species and 17 undetermined species, while samples classified in the lower region (LR) consisted of 11 Panulirus species and 2 undetermined species.

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Published

2019-08-09

How to Cite

Farajallah, A., Tirtaningsih Handayani, B. K., & Wardiatno, Y. (2019). The Suitable COI Marker for Lobster of Genus Panulirus: COI Markers for Panulirus Lobster. Biological Sciences - PJSIR, 62(2), 111–115. https://doi.org/10.52763/PJSIR.BIOL.SCI.62.2.2019.111.115

Issue

Vol. 62 No. 2 (2019): Pakistan Journal of Scientific and Industrial Research Series B: Biological Sciences

Section

Articles