Phylogenetic and Computational Structure-Function Studies of ?-1,4-Glucosidase (Maltase) From Baker’s Yeast (Saccharomyces cerevisiae)

Authors

  • Liaquat Sultana * Food and Marine Resources Research Centre, PCSIR Laboratories Complex, Karachi - 75280, Pakistan
  • Mushtaq Hussaina Food and Marine Resources Research Centre, PCSIR Laboratories Complex, Karachi - 75280, Pakistan
  • Javed A. Qureshi HEJ Research Institute of Chemistry, International Centre for Chemical and Biological Sciences, University of Karachi, Karachi - 75270, Pakistan
  • Shagufta A. Shaikh Food and Marine Resources Research Centre, PCSIR Laboratories Complex, Karachi - 75280, Pakistan

Keywords:

, baker’s yeast, protein homology modelling

Abstract

Study, through blasting, cladogenesis, multiple sequence alignment and protein homology modeling, of

a,1,4 glucosidase from S. cerevisiae YJM789, showed the presence of different paralogues and orthologues of maltase in different genera of fungi and prokaryotes. The sequences of glucosidases contained 4 characteristic consensus regions. In the tertiary structure (modelled) of Baker’s yeast maltase, all the residues of consensus region were congregated in the central region of the folded protein, rendering the formation of catalytic groove. On the basis of the orientation and spatial location of residues in catalytic groove, Asp200 is proposed to be the second substrate-binding site.

Downloads

Published

2008-08-27

How to Cite

Sultana, L., Hussaina, M., Qureshi, J. A., & Shaikh, S. A. (2008). Phylogenetic and Computational Structure-Function Studies of ?-1,4-Glucosidase (Maltase) From Baker’s Yeast (Saccharomyces cerevisiae). Biological Sciences - PJSIR, 51(4), 185–190. Retrieved from https://v2.pjsir.org/index.php/biological-sciences/article/view/666