Regeneration and Acclimatization of Salt-Tolerant Arachis hypogaea Plants Through Tissue Culture

Authors

  • Ejaz Gul Ghauri PCSIR Laboratories Complex, P.O. Peshawar University, Jamrud Road, Peshawar-25120, Pakistan

Keywords:

: tissue culture, Arachis hypogaea, salt-tolerant plant, in vitro regeneration, salt-tolerant peanuts

Abstract

. Excised embryos of Arachis hypogaea were cultured on Murashige and Skoog’s medium (MS medium) supple- mented with different combinations of growth hormones. The highest frequency of callus proliferation (80%) was recorded on MS medium mixed with 1.0 mg/l of 2,4-D and 0.5 mg/l of BAP. These cultures were treated with 0.65 mg/l of trans-4- hydroxy-L-proline (HyP) and various concentrations (0.1-0.5%) of NaCl. In all cases the presence of salt reduced the fresh mass of callus. Shoot regeneration in the cultures took place when transferred to MS medium supplemented with 1.0 mg/l of kinetin (Kin) and 0.5 mg/l of 6-benzylaminopurine (BAP). Percentage of shoot regeneration decreased with the increase of NaCl (0.1- 0.5%) in the shoot regeneration medium. Root formation in these cultures took place when the cultures were nurtured on MS medium free of growth hormones. Regeneration, hardening and acclimatization of the salt- tolerant plants was conducted.

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Published

2006-06-26

How to Cite

Ghauri, E. G. (2006). Regeneration and Acclimatization of Salt-Tolerant Arachis hypogaea Plants Through Tissue Culture. Biological Sciences - PJSIR, 49(3), 203–210. Retrieved from https://v2.pjsir.org/index.php/biological-sciences/article/view/1151