Quantitative Determination of Sinensetin in Orthosiphon stamineus Leaves by Thin-Layer Chromatography and Imaging Densitometry

Short Communication

Authors

  • M. Amzad Hossain Chemistry Division, Atomic Energy Centre, GPO Box No. 164, Ramna, Dhaka-1000, Bangladesh
  • Zakri Zakaria School of Pharmaceutical Sciences, University Sains Malaysia, 11800 Pulau Pinang, Malaysia
  • Zhari Ismaila School of Pharmaceutical Sciences, University Sains Malaysia, 11800 Pulau Pinang, Malaysia

Keywords:

Orthosiphon stamineus, sinensetin quantification, sinensetin extraction, reflectance densitometry

Abstract

An analytical method for the determination of sinensetin in Orthosiphon stamineus leaves by a thin-layer chroma- tography-imaging densitometric method was developed. The procedure consisted of extraction of dry leaf powder with 50% methanol and high performance preparative thin layer chromatography (HPTLC). HPTLC was performed on silica gel plate, using chloroform-ethylacetate (60:40) as the developing solvent for sinensetin. The plate was scanned with a reflectance densitometer at 190 nm. The quantification was done by the external standard method.

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Published

2005-04-25

How to Cite

Hossain, M. A., Zakaria, Z., & Ismaila, Z. (2005). Quantitative Determination of Sinensetin in Orthosiphon stamineus Leaves by Thin-Layer Chromatography and Imaging Densitometry: Short Communication. Biological Sciences - PJSIR, 48(2), 108–109. Retrieved from https://v2.pjsir.org/index.php/biological-sciences/article/view/1351

Issue

Vol. 48 No. 2 (2005): Pakistan Journal of Scientific and Industrial Research

Section

Articles