PARTIAL PURIFICATION AND CHARACTERIZATION OF GLYCOLIPID ISOLATED FROM COLLETOTRICHUM LINDEMUTHIANUM

Authors

  • Fatima Bia PCSIR Laboratories Complex, Karachi - 75280, Pakistan
  • Paul Finchb RHBNC, University of London, Egham, Surrey TW20 OEX, UK

Keywords:

Colletotrichum lindemuthianum, Elicitor, Fatty acids, Carbohydrate.

Abstract

18:1

 

Colletotrichum lindemuthianum, the causative agent of anthracnose in beans (Phaseolus vulgaris) was successfully cul- tured in a complex medium of glucose/neopeptone. The extracellular High Molecular Weight Culture Filtrate Elicitor (HMWCFE) was isolated by simultaneous dialysis and ultrafiltration of nominal cutoff 30,000 dalton cartridge systems. Chromatographic separation provided two fractions Partially Purified Fraction, PPF-I (Mr 2000,000) and PPF-II (Mr < 40,000). Extraction with organic solvents showed that lipid was found in crude preparations. Methanolysis of PPF-I and II analyzed by GC, provided a series of fatty acids characterized by GC-MS viz: behenic (C22), arachidic (C20), isostearic (C18), oleic (C9 ), palmitic (C16), myristic (C14) and capric (C10) acids. Not all the lipid material was released under mild basic hydrolyzing conditions indicating the presence of some stable linkages between lipid and sugar moieties.

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Published

2004-08-30

How to Cite

Bia, F., & Finchb, P. (2004). PARTIAL PURIFICATION AND CHARACTERIZATION OF GLYCOLIPID ISOLATED FROM COLLETOTRICHUM LINDEMUTHIANUM. Biological Sciences - PJSIR, 47(4), 292–297. Retrieved from https://v2.pjsir.org/index.php/biological-sciences/article/view/1537

Issue

Vol. 47 No. 4 (2004): Pakistan Journal of Scientific and Industrial Research

Section

Articles