MICROBIAL SIDE-CHAIN DEGRADATION OF PROGESTERONE I: OPTIMIZATION OF THE TRANSFORMATION CONDITIONS

Authors

  • Nehad Z Adham National Research Centre, Dokki, Giza, Egypt
  • Osama M El- Tayeb Microbiology Department, Faculty of Pharmacy, Cairo University, Egypt
  • Abdel-Gawad M Hashem Microbiology Department, Faculty of Pharmacy, Cairo University, Egypt
  • Heba A El- Refai National Research Centre, Dokki, Giza, Egypt
  • Lotfy A Sallam National Research Centre, Dokki, Giza, Egypt

Keywords:

: Progesterone , Fusarium dimerum, Bioconversion , Androgens.

Abstract

 

The microbial side-chain degradation of progesterone  for  the  production  of  C-19  androgens  was  investigated.  Thirty seven locally isolated fungal cultures were screened for their ability to degrade the side-chain of progesterone. Fusarium dimenim showed the greatest bioconversion efficiency and was selected for further studies. 50% of  the  substrate  was converted to androstenedione after 24 hrs. 72 hrs old culture was able to prod uce maximum yields of testosterone, androstenedione and androstadienedione . The  maximum  conversion  activities  (90%)  of  progesterone  were recorded  at pH 7. The capacity of the fungu s to degrade the side-chain of progesterone was greatly dimini shed on using high concentration  of progesterone . The bioconversion  estimates  sharply decreased  by using gl ucose  syrup, com steep liquor  and  glucose-com  steep media.

Downloads

Published

2002-08-26

How to Cite

Adham, N. Z., Tayeb, O. M. E.-., Hashem, A.-G. M., Refai, H. A. E.-., & Sallam, L. A. (2002). MICROBIAL SIDE-CHAIN DEGRADATION OF PROGESTERONE I: OPTIMIZATION OF THE TRANSFORMATION CONDITIONS. Biological Sciences - PJSIR, 45(4), 250–255. Retrieved from https://v2.pjsir.org/index.php/biological-sciences/article/view/1801

Issue

Vol. 45 No. 4 (2002): Pakistan Journal of Scientific and Industrial Research

Section

Articles