HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC DETERMINATION OF HYDRAZINE IN WATER USING 2-HYDROXYNAPHTHALDEHYDE AS A DERIVATIZING REAGENT

Authors

  • M Y Khuhawar Dr M A Kazi Institute of Chemistry,University of Sindh, Jamshoro, Sindh, Pakistan
  • A D Rajper Dr M A Kazi Institute of Chemistry,University of Sindh, Jamshoro, Sindh, Pakistan
  • F M ARind Dr M A Kazi Institute of Chemistry, University of Sindh, Jamshoro, Sindh, Pakistan

Keywords:

: Hydrazine derivation, 2-Hydroxynaphthaldehyde, Liquid chromatography.

Abstract

Hydrazine (HZ) derivative with 2-hydroxyn aphthaldehyde(HN) eluted with methanol: acetonitrile: water (95: 1:4 v/v/v) from reverse phase HPLC column Microsorb C-18, 5µm with  UV  detection  at  330nm.  Hydrazine,  thiosemicarbazide (TSC) and phenylthiosemicarbazide (PTSC) derivatives of HN also separated when eluted with methanol: Chloroform: acetonitrile: water (65:4 :2:29 V/V/V/V) with a flow rate 1 ml/minute, with detection limit in a  ran ge 0.54  to  16 ng/ injection (10µ1). Linear calibration curve for HZ was obtainedwith 0.54-2 .7µg/ml. The effect of variables on the determination  of HZ was  examined  and  method  has been  proposed  for  the determination  of HZ  from water.

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Published

2002-12-23

How to Cite

Khuhawar, M. Y., Rajper, A. D., & ARind, F. M. (2002). HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC DETERMINATION OF HYDRAZINE IN WATER USING 2-HYDROXYNAPHTHALDEHYDE AS A DERIVATIZING REAGENT. Biological Sciences - PJSIR, 45(6), 371–374. Retrieved from https://v2.pjsir.org/index.php/biological-sciences/article/view/1840

Issue

Vol. 45 No. 6 (2002): Pakistan Journal of Scientific and Industrial Research

Section

Articles