?MONOSACCHARIDE COMPOSITION OF HIGH MOLECULAR WEIGHT CULTURE FILTRATE ELICITOR OF COLLETOTRICHUM LINDEMUTHIANUM, DETERMINED BY HIGH PERFORMANCE ANION-EXCHANGE/PULSED AMPEROMETRIC DETECTION (HPAE-PAD)
Keywords:
Colletotrichum lindemuthianum, Elicitor, Monosaccharide composition.Abstract
Monosaccharide composition of crude and partially purified high molecular weight culture filtrate elicitor (HMWCFE)
fractions-I and II isolated by molecular sieve chromatography from Colletotrichutn lindemuthianum 1M! (112166)
isolate were acid hydrolysed for 0.5, 1.5, 3 and 5 h. Liberated monosaccharides were analysed directly using high
performance anion-exchange chromatography with pulsed amperometric detection (HPAE-PAD) Mannose galactose
were found as major sugars of each sample whereas glucosamine, arabinose and rhamnose were additional sugars.
Glucosamine was found as a major sugar (26% w/w) of Fraction-I and first time reported from preparations of this
organism.