?ABUNDANCE, DISTRIBUTION AND DIVERSITY OF BACILLUS THURINGIENSIS IN PAKISTANIAN ENVIRONMENT

Authors

  • ESTHER KHAN Centre for Advanced Molecular Biology, University of the Punjab, Canal Bank Road,Thokar Niaz Baig, Lahore-53700, Pakistan
  • SHAHID KARIM , Centre for Advanced Molecular Biology, University of the Punjab, Canal Bank Road,Thokar Niaz Baig, Lahore-53700, Pakistan
  • RAHAT MAKHDOOM Centre for Advanced Molecular Biology, University of the Punjab, Canal Bank Road,Thokar Niaz Baig, Lahore-53700, Pakistan
  • SHEIKH RlAZUDDIN Centre for Advanced Molecular Biology, University of the Punjab, Canal Bank Road,Thokar Niaz Baig, Lahore-53700, Pakistan

Keywords:

Crystal protein, Lepidoptera, Diptera, Bacillus thuringiensis.

Abstract

Four hundred and seventy six samples of soil, insect cadavers, wild animal dung, decaying leaves and grain dust
collected from different ecological environments in Pakistan, were used as source material to separate 647 Bacillus
thuringiensis isolates. The abundance and frequency of Bt in the various samples was found to be in the order of
soil> grain dust> wild animal dung> bird droppings> others. Isolates were randomly selected to study pesticidal
protein profile on SDS-PAGE and entomocidal properties in biotoxicity assays against Heliothis armigera
(Lepidoptera) and Anophelese stephensi (Diptera). Several isolates were more efficacious against Heliothis armigera
than the available reference strain.

Published

1995-06-19

How to Cite

KHAN, E., KARIM, S., MAKHDOOM, R., & RlAZUDDIN, S. (1995). ?ABUNDANCE, DISTRIBUTION AND DIVERSITY OF BACILLUS THURINGIENSIS IN PAKISTANIAN ENVIRONMENT. Biological Sciences - PJSIR, 38(5-6), 192–195. Retrieved from https://v2.pjsir.org/index.php/biological-sciences/article/view/2471