?LOCAL DEVELOPMENT OF TSH RADIO IMMUNOASSAY AND IT'S EVALUATION

Authors

  • KHAN MOHAMMAD SAJIO Atomic Energy Medical Centre, P.O. Box 377, Multan, Pakistan
  • MAHFOOz AKHTAR Atomic Energy Medical Centre, P.O. Box 377, Multan, Pakistan
  • RIFFAT PARVEEN Atomic Energy Medical Centre, P.O. Box 377, Multan, Pakistan
  • NAHEED ANWAR Atomic Energy Medical Centre, P.O. Box 377, Multan, Pakistan

Keywords:

Development,TSH. Evaluation.Thyroid.Thyroid function.

Abstract

Pakistan isan iodine deficientcountry,Thyroiddiseasesare verycommon andtheir investigation requires heavy
foreign exchange to import relevant commercial kits. AEMC, Multan therefore tried to develop adequate and eco-
nomical protocol for TSH radioimmunoassay.TSH-RIAprotocol supplied by the commercial source did not provide
the desired quality and evaluation of thyroid diseases.Our method was therefore optimized using precision profile as
the criteria of performance.Atotal of sixassay systemswerestudied,out of which the most precise one was selected.
The new systemgives resultswithinexpectedlimits.The sensitivityof thesystem,0.3~lU/ml is suitable for low dose
measurements. The working range, 0.6-25.1 ~lU/ml covers hypo, hyper and euthyroid levels of the hormone. Com-
parison with other laboratoriesshows an agreementof values.Alowcost, locallydeveloped good qualityTSH RIAis
therefore in our hand.

Published

1995-06-19

How to Cite

SAJIO, K. M., AKHTAR, M., PARVEEN, R., & ANWAR, N. (1995). ?LOCAL DEVELOPMENT OF TSH RADIO IMMUNOASSAY AND IT’S EVALUATION. Biological Sciences - PJSIR, 38(5-6), 205–210. Retrieved from https://v2.pjsir.org/index.php/biological-sciences/article/view/2474

Issue

Vol. 38 No. 5-6 (1995): Pakistan Journal of Scientific and Industrial Research

Section

Articles

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