?HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC AND POLAROGRAPHIC METHODS FOR THE QUANTITATION OF NICARDIPINE .HYDROCHLORIDE IN CAPSULES

Authors

  • A. A. MUSTAFA Department of Pharmaceutical Chemistry, College of Pharmacy, P.O. Box 2457, King Saud University, Riyadh-11451, Saudi Arabia
  • K. A. AL- RASHOOD Department of Pharmaceutical Chemistry, College of Pharmacy, P.O. Box 2457, King Saud University, Riyadh-11451, Saudi Arabia
  • M. E. M. HAGGA Department of Pharmaceutical Chemistry, College of Pharmacy, P.O. Box 2457, King Saud University, Riyadh-11451, Saudi Arabia
  • E. A. GAO- KARIEM Department of Pharmaceutical Chemistry, College of Pharmacy, P.O. Box 2457, King Saud University, Riyadh-11451, Saudi Arabia
  • M. E. AL AWAOY Department of Pharmaceutical Chemistry, College of Pharmacy, P.O. Box 2457, King Saud University, Riyadh-11451, Saudi Arabia

Keywords:

Capsules, Quantitation.

Abstract

Nicardipine hydrochloride in bulk and in capsule dosage form has been assayed by two proposed independent
analytical methods; (i) isocratic reverse-phase high-performance liquid chromatography (RP-HPLC) and (ii) differen-
tial pulse polarography (DPP). The RP-HPLC involves employment of u-Bondapak-Ci , column of 300 x 3.9mm i.d.
dimensions and mobile phase composed of 80% vtv CH3 CN +20% vIv (0.0 1M) CH3COONa buffer adjusted to pH 3.5
with glacial CH3COOH. The flow rate of the eluent was maintained at 1.5 ml/min. In the polarographic method,
nicardipine produces a distinct reduction wave and a diffusion-controlled current which is linearly related to
concentration ofnicardipine HCl over the range 10-100 ~g/ml. The RP-HPLC and DPP methods yielded comparable
results when applied for the assay of nicardipine HCl in capsules (Pycarden®/30mg). However, the sensitivity and
selectivity of the RP-HPLC can be improved substantially if the detection is carried out amperometrically.

Published

1994-01-24

How to Cite

MUSTAFA, A. A., RASHOOD, K. A. A.-., HAGGA, M. E. M., KARIEM, E. A. G.-., & AWAOY, M. E. A. (1994). ?HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC AND POLAROGRAPHIC METHODS FOR THE QUANTITATION OF NICARDIPINE .HYDROCHLORIDE IN CAPSULES. Biological Sciences - PJSIR, 37(1-2), 1–4. Retrieved from https://v2.pjsir.org/index.php/biological-sciences/article/view/2558

Issue

Vol. 37 No. 1-2 (1994): Pakistan Journal of Scientific and Industrial Research

Section

Articles