Short Communication Optimization of Substrate Concentration for Enhanced Citric Acid Production by Aspergillus niger M-101

Short Communication: Citric Acid Production by A. niger

Authors

  • Aftab Nadeem Nadeem Institute of Molecular Biology and Biotechnology, The University of Lahore, Lahore, Pakistan
  • Saghir Ahmad Jafri Institute of Molecular Biology and Biotechnology, The University of Lahore, Lahore, Pakistan
  • Shahjahan Baig Food Biotechnology Research Center, PCSIR Laboratories Complex, Shahrah-e-Jalaluddin Roomi, Lahore, Pakistan
  • Muhammad Irfan Food Biotechnology Research Center, PCSIR Laboratories Complex, Shahrah-e-Jalaluddin Roomi, Lahore, Pakistan
  • Quratulain Syed Food Biotechnology Research Center, PCSIR Laboratories Complex, Shahrah-e-Jalaluddin Roomi, Lahore, Pakistan

Keywords:

Aspergillus niger, citric acid, kinetic parameters, beet molasses, submerged fermentation

Abstract

Studying the effect of different sugar concentration of beet molasses on citric acid accumulation in batch fermentation, 150 g/litre sugar concentration was found to be optimal for maximum citric acid production (27.25±2.35 g/ litre) using Aspergillus niger M-101. Yp/x value for product formation increased with increase in sugar concentration through out the study. Qp value for citric acid production also increased with increase in sugar concentration and reached the maximum (0.141 g/1itre/h) at 150 g/litre sugar concentration but with further increase in sugar concentration, the value decreased. When culture was grown at different substrate concentrations, the kinetic parameters monitored for Yx/s, Yp/s and Yp/x, Qp, Qs and qp showed significant enhancement (p £ 0.05) in citric acid production as well as biomass growth.

 

 

 

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Published

2009-10-26

How to Cite

Nadeem, A. N., Jafri, S. A., Baig, S., Irfan, M., & Syed, Q. (2009). Short Communication Optimization of Substrate Concentration for Enhanced Citric Acid Production by Aspergillus niger M-101: Short Communication: Citric Acid Production by A. niger. Biological Sciences - PJSIR, 52(5), 275–277. Retrieved from https://v2.pjsir.org/index.php/biological-sciences/article/view/564

Issue

Vol. 52 No. 5 (2009): Pakistan Journal of Scientific and Industrial Research

Section

Articles