Alkaline Protease Production from Industrial Waste by Bacillus subtilis ML-4: Alkaline Protease Production from Industrial Waste

Muhammad Gul Sher; Muhammad Nadeem; Quratulain Syed; Muhammad Irfan; Shahjahan Baig

Authors

Muhammad Gul Sher Food and Biotechnology Research Centre, PCSIR Laboratories Complex, Lahore - 54600, Pakistan
Muhammad Nadeem Food and Biotechnology Research Centre, PCSIR Laboratories Complex, Lahore - 54600, Pakistan
Quratulain Syed Food and Biotechnology Research Centre, PCSIR Laboratories Complex, Lahore - 54600, Pakistan
Muhammad Irfan Food and Biotechnology Research Centre, PCSIR Laboratories Complex, Lahore - 54600, Pakistan
Shahjahan Baig Food and Biotechnology Research Centre, PCSIR Laboratories Complex, Lahore - 54600, Pakistan

Keywords:

protease production, B. subtilis, poultry feed, detergent formulation

Abstract

The influence of various culture conditions on protease production by Bacillus subtilis ML-4 was studied in the presence of growth medium containing poultry feed waste (5%), K2HPO4 (0.3%), CaCl2 (0.03%) and MgSO4 (0.015%). Maximum protease production (264.25 ± 1.86 U/ml) was observed at initial pH 9 with 3% (v/v) of inoculum size after 48 h of incubation at 37 °C. The alkaline protease was stable over a broad range of temperature (30 to 60 °C) and pH (8 to 11). However, maximum activity (155.45 U/ml) was observed at temperature 50 °C and pH 10.

Keywords:

Alkaline Protease Production from Industrial Waste by Bacillus subtilis ML-4