Improvement of Amylase Production by UV Mutagenesis of Aspergillus flavus FSS63 under Solid State Fermentation

Amylase Production by Aspergillus flavus

Authors

  • Yasser Bakri Department of Molecular Biology and Biotechnology, Atomic Energy Commission of Syria, P.O. Box 6091, Damascus, Syria
  • Samir Elkhouri Department of Molecular Biology and Biotechnology, Atomic Energy Commission of Syria, P.O. Box 6091, Damascus, Syria

DOI:

https://doi.org/10.52763/PJSIR.BIOL.SCI.59.2.2016.80.84

Keywords:

Aspergillus flavus, amylase, ultraviolet radiation

Abstract

Enhancement of the amylase productivity by Aspergillus flavus was investigated. Spores of
strain were exposed to ultraviolet (UVC) radiation and 10 different mutants were selected and isolated
from starch plate agar on the basis of the visible clearance zone around the colonies. The amylase production
by selected mutants was evaluated under solid state fermentation. One mutant of A. flavus FSS63UV8
showed higher biosynthesis level of amylase (733 IU/g), which was 3.35 fold higher than that detected
in the parental strain. Physical parameters optimisation revealed that the optimum pH and temperature for
amylase production obtained by mutant are 7.0 and 35°C, respectively. Among several tested agricultural
wastes , wheat bran was found to support the highest yield of amylase after 5 days of incubation. A. flavus
FSS63UV8 strain proved to be a promising microorganism for a high amylase production in a simple
medium.

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Published

2016-08-24

How to Cite

Bakri, Y., & Elkhouri, S. (2016). Improvement of Amylase Production by UV Mutagenesis of Aspergillus flavus FSS63 under Solid State Fermentation: Amylase Production by Aspergillus flavus. Biological Sciences - PJSIR, 59(2), 80–84. https://doi.org/10.52763/PJSIR.BIOL.SCI.59.2.2016.80.84

Issue

Vol. 59 No. 2 (2016): Pakistan Journal of Scientific and Industrial Research Series B: Biological Sciences

Section

Articles