SDS-PAGE OF HAPTENISED MOUSE SKIN MICROSOMAL PROTEIN

Authors

  • L Khan PCSIR Laboratories, Peshawar-25100 , Pakistan
  • N Shafi pcsIR Laboratories, Peshawar-25100 , Pakistan
  • R J Schmidt Welsh School of Pharmacy, UWCC, P O Box 13, Cardiff CFI 3XF, United Kingdom

Keywords:

Hapten, Microsomal Proteins, SDS-PAGE.

Abstract

As an approach to resolve the haptenised proteins involved in the production of dermatoses 2, 4-dinitrochlorobenzene and 2, 4-dinitrofluorobenzene were chosen as model haptens with a well established and understood mechanism of protein preactivity. These haptens were applied to mouse skin in Vivo. Prior to sacrifice after 6 days, control and treated skin was homogenized and the microsomal proteins examined by dialysis, gel permeation and SDS-PAGE. Rather unexpectedly only one protein band in DNFB treated skin, appeared to have become labelled with an apparent molecul ar mass of 47,000 Da.

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Published

2001-04-23

How to Cite

Khan, L., Shafi, N., & Schmidt, R. J. (2001). SDS-PAGE OF HAPTENISED MOUSE SKIN MICROSOMAL PROTEIN. Biological Sciences - PJSIR, 44(2), 83–85. Retrieved from https://v2.pjsir.org/index.php/biological-sciences/article/view/1884